ISAba1 targets a specific position upstream of the intrinsic ampC gene of Acinetobacter baumannii leading to cephalosporin resistance
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چکیده
منابع مشابه
ISAba1 targets a specific position upstream of the intrinsic ampC gene of Acinetobacter baumannii leading to cephalosporin resistance.
Sir, Resistance to third-generation cephalosporins such as ceftazidime and cefotaxime in Acinetobacter baumannii is often due to increased expression of an intrinsic ampC gene. Expression increases when the ISAba1 insertion sequence (IS) is present in the appropriate orientation upstream of the ampC gene, providing an outward-facing promoter that appears to be stronger than the intrinsic promot...
متن کاملTn6168, a transposon carrying an ISAba1-activated ampC gene and conferring cephalosporin resistance in Acinetobacter baumannii.
OBJECTIVES To explore the cause of third-generation cephalosporin resistance in Australian Acinetobacter baumannii isolates belonging to global clone 1 (GC1). METHODS GC1 isolates from Australia were tested for resistance to ceftazidime and cefotaxime using disc diffusion and MICs. PCR was used to determine the context of ISAba1-ampC configurations and amplicons were sequenced. The level of t...
متن کاملHorizontal transfer of an ISAba125-activated ampC gene between Acinetobacter baumannii strains leading to cephalosporin resistance.
Sir, In Acinetobacter baumannii, resistance to third-generation cephalosporins such as ceftazidime and cefotaxime is known to arise as a consequence of acquisition of an insertion sequence, ISAba1, upstream of the chromosomal ampC gene. Indeed, ISAba1 is frequently found upstream of the ampC gene in isolates that are resistant to third-generation cephalosporins, and the promoter that directs tr...
متن کاملcorrelation between isaba1 upstream ampc gene and resistance to cefotaxime in acinetobacter baumannii: a serious threat to nosocomial infections
conclusions isaba1 has become an important factor in a. baumannii’s resistance to cefotaxime. results isaba1 located upstream of blaampc was found in 24 (48%) of the a. baumannii isolates. in all of the studied isolates that had isampc, the mic for cefotaxime was 64 - 256 μg/ml. based on the rep-pcr patterns among the resistant isolates, the highest number of isampc positive isolates belonged t...
متن کاملIdentification of fluoroquinolone-resistant extended-spectrum β-lactamase (CTX-M-8)-producing Escherichia coli ST224, ST2179 and ST2308 in buffalo (Bubalus bubalis).
D2 differed from one another by fewer than five single nucleotide differences, but only the WM98 sequence was not interrupted by large insertions or deletions (positions of insertions/deletions are indicated in Figure 1). AB307-0294 (GenBank accession number CP001172) was also identical over most of this span, but contained patches that differed and lacked a large span. The AB0057 sequence (Gen...
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ژورنال
عنوان ژورنال: Journal of Antimicrobial Chemotherapy
سال: 2013
ISSN: 0305-7453,1460-2091
DOI: 10.1093/jac/dkt233